Allexis cauliflora is a Cameroonian medicinal plant belonging to the violaceae family. It is used in traditional medicine to treat fever, syphilis and malaria. The aim of this study is to screen the peptides from the leaves of Allexis cauliflora and assess their biological activities. Previous studies have demonstrated antimicrobial properties of this plant. These properties have been mainly attributed to phenolic compounds. However, other plant defense molecules, such as antimicrobial peptides, may be present. In this work, we studied peptide extracts from the leaves of Allexis cauliflora. This plant has also been examined to determine its antimicrobial activities and its minimum inhibitory concentrations against bacteria pathogenic to humans and fungi using the agar well diffusion method. The corresponding concentration value antilog was taken as the minimum inhibitory concentration value. All values of test results were expressed as triple means, mean ± standard deviation. The extract from the leaves of this plant has shown antimicrobial activity against the bacterial and fungal pathogens studied. The phytochemical screening carried out showed the presence of flavonoids and triterpenes. Based on the results obtained, the medicinal value of this plant could be attributed to the presence of secondary metabolites in traditional medicinal plants.