Biosynthesis of silver nanoparticles (AgNPs) was achieved by a novel, simple green chemistry procedure. The effect of time on the synthesis of silver nanoparticles was carried out at room temperature, at 10 min, 30 min, 1 hr and 2 hrs. The successful formation of silver nanoparticles has been confirmed by UV–vis, FTIR, XRD, and SEM analysis. In vitro antibacterial activity against Escherichia coli, Pseudomonas aeruginosa, Klebsiella planticola (Gram-negative), and Staphylococcus pneumonia, Bacillus subtillus, Entrococcus caccae, lactobacillus acidophilus (Gram-positive) has been studied by agar diffusion method and compared between three standard antibiotic ampicillin (AMP), chloramphenicol (CHL) and tetracycline (TET). The maximum zone of inhibition was observed in the Au NPs against E. coli, (31.00±0.00 mm), K. planticola (20.00±0.00 mm), B subtillus (19.00±0.00 mm) and E caccae (18.00±0.00 mm). In vitro antioxidant activity was carried out using DPPH method with 3.125 to 100 μg/mL concentration of silver nanoparticles. The in vitro antioxidant activity was found to show biphasic response and inhibitory activity was found between 100 to 25 μg/mL followed by stimulatory activity from 12.5 to 3.25 μg/mL which was not significant. It can be concluded silver nanoparticles synthesized by green synthesis using possess antioxidant activity and act as an effective antibacterial agent.

Keywords: Silver Nanoparticles; Antibacterial Activity; Antioxidant Activity; L. trichilioïdes;