A novel naphthalene based Schiff base ligand (SBL) was synthesized by the condensation of naphthalene -1-8-diamine with 4-dimethylamino benzaldehyde as a colorimetric probe for the detection of Fe(III) ions selectively in ethanol solution. UV-visible spectroscopic technique was employed to study the sensing ability of probe SBL for Fe(III)ions and monitored in ethanol solution (pH 4).The Jobs plot confirmed 1:1 SBL to Fe(III)molar complex formation. DFT computation were also supported the binding framework between SBL and Fe(III).The binding constant for this complex is with detection limit of 6.925X 10⁴ M. Cyclic voltammetry study shows that there is no change in electrochemical structure of SBL after complex formation of SBL with Fe(III). The core finding of this research is that both the SBL and the SBL:Fe complex exhibited significant antibacterial activity, specifically effective against gram-negative bacterial strains. These results highlight the potential of this synthesized Schiff base framework as a novel, dual purpose compound with applications not only in metal ion sensing but also as a promising candidate for antimicrobial agent in biological system.