Acridinedione has attracted significant interest in the fields of material science, drug development, and chemical and medicinal chemistry. Recent research on the synthesis of acridine-1,8-dione derivatives, including cyclocondensation and multicomponent reactions, is included in this review. These substances have been used to make antibacterial, anticancer, and anti-inflammatory medications because of their strong biological activity. They serve as fluorescence probes in material science as well. The main objectives of recent developments have been to create more effective synthesis methods for acridine-1,8-diones and investigate new uses; and these approaches are discussed here.