Morus nigra L., a widely distributed medicinal plant, is increasingly recognized for its rich phytochemical profile and potential therapeutic applications. The present study focuses on the systematic isolation, characterisation, and biological evaluation of phytochemicals present in the leaves of Morus nigra L. Crude extracts were prepared using solvents of varying polarity, followed by fractionation through chromatographic techniques such as column chromatography and TLC. Major classes of secondary metabolites, including proteins, carbohydrates, phenolics, tannins, flavonoids, saponins, glycosides, steroids, terpenoids, alkaloids, were identified. Structural characterisation of isolated compounds was accomplished using spectroscopic methods, including FTIR, ¹HNMR, ¹³C NMR and GC–MS. Overall, the findings highlight Morus nigra L. as a promising natural source of bioactive compounds.