Medicinal plants are of great importance to the health of individuals and communities in general. The vital role of plants lies in some chemical substances that produce a definite physiological action on the human body. The different extracts of Urospermum picroides were tested against the following bacteria; six gram negative bacteria: Proteus vulgaris, Klebsiella pneumoniae, Shigella, Escherichia coli, Erwinia carotovora, Pseudomonas aeruginosa, and three gram positive bacteria; Streptococcus pyogenes, Staphylococcus aureus and Bacillus subtilis. The phytochemical analysis of the aerial parts of U. picroides indicated that the plant is rich in secondary compounds. Hexane extract had the highest scavenging activity (1.54 mg/mL). Methanol and ethyl acetate extracts of U. picroides showed the broad spectrum against the tested bacteria. Whereas, hexane extracts expressed an activity against E. coli and Erwinia carotovora (inhibition zone = 6.5 and 7 mm, respectively). Petroleum ether extract showed the inhibitory activities against Escherichia coli, Klebsiella pneumoniae, Erwinia carotovora and Candida albicans (inhibition zone =7, 8, 6.5 and 6.5 mm, respectively). The pathogen Staphylococcus aureus and Bacillis subtillis were the most sensitive bacteria (inhibition zone=10 mm, each) in case of ethyl acetate extract. On the other hand, Erwinia carotovora and Candida albicans were the most sensitive organisms to different extracts.